Middle East Respiratory Syndrome Coronavirus Nucleic Acid
Pangalan ng Produkto
HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)
Epidemiology
Middle East respiratory syndrome coronavirus (MERS-CoV), isang β-coronavirus na nagdudulotrespiratory disease sa mga tao, ay unang natukoy sa isang namatay na 60 taong gulang na Saudi Arabian male patient noong ika-24 ng Hulyo, 2012. Ang klinikal na presentasyon ng MERS-CoV infection ay mula sa asymptomatic state o mild respiratory symptoms hanggang sa matinding acute respiratory disease kahit kamatayan.
Channel
FAM | MERS virus RNA |
VIC(HEX) | Panloob na Kontrol |
Mga Teknikal na Parameter
Imbakan | ≤-18℃ Sa dilim |
Shelf-life | 9 na buwan |
Uri ng Ispesimen | Mga bagong nakolektang pamunas ng nasopharyngeal |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1000 Kopya/mL |
Pagtitiyak | Walang cross-reactivity sa human coronaviruses human coronavirus SARSr-CoV at iba pang karaniwang pathogens. |
Mga Naaangkop na Instrumento: | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Daloy ng Trabaho
Pagpipilian 1.
Inirerekomendang extraction reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction o Purification Reagent (YDP315-R) ng Tiangen Biotech (Beijing) Co., Ltd.
Opsyon 2.
Inirerekomendang extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) at Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).