12 Uri ng Respiratory Pathogen
Pangalan ng produkto
HWTS-RT071A 12 Uri ng Respiratory Pathogen Nucleic Acid Detection Kit (Fluorescence PCR)
Channel
| Channel | hu12Buffer ng Reaksyon A | hu12Buffer ng Reaksyon B | hu12Buffer ng Reaksyon C | hu12Buffer ng Reaksyon D |
| FAM | SARS-CoV-2 | HADV | HPIV Ⅰ | HRV |
| VIC/HEX | Panloob na Kontrol | Panloob na Kontrol | HPIV Ⅱ | Panloob na Kontrol |
| CY5 | IFV A | MP | HPIV Ⅲ | / |
| ROX | IFV B | RSV | HPIV Ⅳ | HMPV |
Mga Teknikal na Parameter
| Imbakan | ≤-18 ℃ |
| Shelf-life | 12 buwan |
| Uri ng Ispesimen | Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2：300 Copy/mLinfluenza B virus：500 Copy/mLinfluenza A virus：500 Copy/mL Adenovirus：500 Copies/mL mycoplasma pneumoniae：500 Kopya/mL respiratory syncytial virus：500 Copy/mL, parainfluenza virus (Ⅰ, Ⅱ, Ⅲ, Ⅳ):500 Copy/mL Rhinovirus：500 Copy/mL human metapneumovirus：500 Copies/mL |
| Pagtitiyak | Ipinapakita ng cross-reactivity na pag-aaral na walang cross-reactivity sa pagitan ng kit na ito at enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella-herpes zoster virus, bordetella pertussis, streptoberculus pyogenes, mycobacterium tuberculosis, tuberculosis. albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans at human genomic nucleic acid. |
| Mga Naaangkop na Instrumento | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Fast Real-Time PCR SystemsQuantStudio®5 Real-Time na PCR System SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd. ) LightCycler®480 Real-Time na PCR system, LineGene 9600 Plus Real-Time PCR Detection System（FQD-96A，Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Daloy ng Trabaho
Pagpipilian 1.
Inirerekomendang extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ng Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ang pagkuha ay dapat isagawa sa mahigpit na alinsunod sa mga tagubilin.
Opsyon 2.
Inirerekomendang extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) at Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3017-32) Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-30006) Micro-Test Med-Tech Co., Ltd., ang pagkuha ay dapat isagawa nang mahigpit ayon sa mga tagubilin. Ang inirerekomendang dami ng elution ay 80μL.
Opsyon 3.
Inirerekomendang extraction reagent: Nucleic Acid Extraction o Purification Kit (YDP315) ng Tiangen Biotech(Beijing) Co.,Ltd., ang pagkuha ay dapat na isagawa ayon sa mga tagubilin nang mahigpit. Ang inirerekomendang dami ng elution ay 100μL.
